[Data set] T7 RNA polymerase-based gene expression from a transcriptionally silent rDNA spacer in the endosymbiont-harboring trypanosomatid Angomonas deanei

Registered ARC content:

• root
  • README.md
  • isa.investigation.xlsx
  • assays
    • S1_1_rDNA-spacers-on-different-chrosomosomes
      • README.md
      • isa.assay.xlsx
    • S2_1_Genomic-map-of-Adea319
      • README.md
      • isa.assay.xlsx
    • S2_2_Verification-of-genomic-integration-by-PCR
      • README.md
      • isa.assay.xlsx
    • S2_3_Verification-of-T7RNAP-production-by-Western-Blot
      • README.md
      • isa.assay.xlsx
      • dataset
        • 2023-07-07 Stain Free Gel.jpg
        • 2023-07-11 Chemiluminescence + Colorimetric.jpg
    • S3_1_Growth-curve-of-WT-and-Adea373-on-blast
      • README.md
      • isa.assay.xlsx
      • dataset
        • Growth curve_Adea373 and WT on blast.pzf
    • S3_2_Growth-curve-of-WT-and-Adea384-on-nours
      • README.md
      • isa.assay.xlsx
      • dataset
        • Growth curve_Adea384 and WT on nours.pzf
    • S3_3_Genomic-maps-of-Adea373-and-Adea384
      • README.md
      • isa.assay.xlsx
    • S4_1_Genomic-map-of-Adea400
      • README.md
      • isa.assay.xlsx
    • S4_2_Verification-of-insertion-by-PCR-I
      • README.md
      • isa.assay.xlsx
      • dataset
        • 2023-06-13 SYBR Safe stained agarose gel.jpg
    • S4_3_Verification-of-insertion-by-PCR-II
      • README.md
      • isa.assay.xlsx
      • dataset
        • 2023-05-31 SYBR Safe stained agarose gel.jpg
    • S4_4_Verification-of-insertion-by-Southern-Blot
      • README.md
      • isa.assay.xlsx
      • dataset
        • 2024-04-11 Chemiluminescence.tif
    • S4_5_Growth-curves-of-WT-Adea319-and-Adea400
      • README.md
      • isa.assay.xlsx
      • dataset
        • Growth curve_WT-Adea319-Adea400.pzf
    • S4_6_Knock-out-strategy-for-T7RNAP
      • README.md
      • isa.assay.xlsx
    • S4_7_Verification-of-knock-out-by-PCR
      • README.md
      • isa.assay.xlsx
      • dataset
        • 2024-03-01 SYBR Safe stained agarose gel.tif
    • S4_8_Growth-curves-of-WT-Adea373-and-Adea442
      • README.md
      • isa.assay.xlsx
      • dataset
        • Growth curve_WT-Adea373-Adea442.pzf
    • S5_1_Genomic-map-of-Adea456
      • README.md
      • isa.assay.xlsx
    • S5_2_Verification-of-insertion-by-Southern-Blot
      • README.md
      • isa.assay.xlsx
      • dataset
        • BclI-SpeI Southern - 2025-01-09 SYBR Safe stained gel.tif
        • BclI-SpeI Southern - 2025-01-13 Chemiluminscence.tif
    • S5_3_Growth-curves-of-WT-and-Adea456
      • README.md
      • isa.assay.xlsx
    • S5_4_Epifluorescence-microscopy-of-Adea126-and-Adea456
      • README.md
      • isa.assay.xlsx
      • dataset
        • Cropped pictures
          • Adea126 DIC (6).tif
          • Adea126 H (6).tif
          • Adea126 mSc (6).tif
          • Adea126 merge (6).tif
          • Adea456 DIC (6).tif
          • Adea456 H (6).tif
          • Adea456 mSc (6).tif
          • Adea456 merge (6).tif
          • WT DIC (6).tif
          • WT H (6).tif
          • WT merge (6).tif
        • Uncropped pictures
          • Adea126 - 6 DIC Adea126-Image Export-03.jpg - 6 H Adea126-Image Export-01.jpg - 6 mS Adea126-Image Export-02.jpg - 6 merge Adea126 Export-04_c1+2+3.jpg
          • Adea456 - 6 DIC Adea456 C4 new-Image Export-12.jpg - 6 H Adea456 C4 new-Image Export-13.jpg - 6 mS Adea456 C4 new-Image Export-14.jpg - 6 merge Adea456 C4 new_c1+2+3.jpg
          • WT - 6 DIC AdeaWt-Image Export-14.jpg - 6 DIC AdeaWt-Image merge-Image Export-01_c1+2+3.jpg - 6 H AdeaWt-Image Export-15.jpg
    • S5_5_Quantification-of-fluorescent-cells
      • README.md
      • isa.assay.xlsx
      • dataset
        • Quantification_mSc strains.xlsx
    • S5_6_In-gel-fluorescence-assay-of-Adea126-and-Adea456
      • README.md
      • isa.assay.xlsx
      • dataset
        • 2025-01-17 Cy3 Filter.tif
        • 2025-01-17 Stainfree gel image.tif
    • S5_7_Quantification-of-in-gel-assay
      • README.md
      • isa.assay.xlsx
      • dataset
        • Quantification_in-gel assay.xlsx
  • studies
    • S1_Identification-of-potentially-silent-loci
      • README.md
      • isa.study.xlsx
    • S2_Heterologous-expression-of-the-T7RNAP
      • README.md
      • isa.study.xlsx
    • S3_Implementation-of-new-antibiotic-resistance-cassettes
      • README.md
      • isa.study.xlsx
    • S4_T7RNAP-driven-expression-from-the-rDNA-spacer
      • README.md
      • isa.study.xlsx
    • S5_Analysis-of-gene-expression-strength
      • README.md
      • isa.study.xlsx