{
"@id": "./",
"filename": "isa.investigation.xlsx",
"identifier": "Kaplunova_2025_TPJ",
"title": "Artificial Soil (ArtSoil): recreating soil conditions in synthetic plant growth media",
"description": "Study described the development of Artificial Soil (ArtSoil), a growth medium containing essential nutrients for plant growth and aqueous soil extract (ASE) to maintain soil microbiomes and edaphic factors, simultaneously eliminating the need for sugar supplementation in the medium. We compared Arabidopsis thaliana grown on conventional media to ArtSoil under various growth conditions, and showed that plants grown on ArtSoil did not exhibit physiological side effects induced by sucrose. We demonstrate an application for ArtSoil in single-cell transcriptomics and report microbiota-induced cell-type specificity in immune and nitrogen signaling. We tested ArtSoil with six types of ASEs to present the potential of ArtSoil in decoupling the effects of nutrients from microbiota in plant growth. We conclude that ArtSoil recapitulates the soil environment compared to conventional media, hence, enabling physiologically relevant plant growth. ",
"people": [
{
"@id": "https://orcid.org/0009-0008-3468-024X",
"firstName": "Vera",
"lastName": "Kaplunova",
"email": "Vera.Kaplunova@hhu.de",
"address": "Universit\u00e4tstr. 1, 40225 D\u00fcsseldorf",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "ORCID",
"value": "https://orcid.org/0009-0008-3468-024X"
}
]
},
{
"@id": "0009-0005-5539-497X",
"firstName": "Houda",
"lastName": "Alioui",
"email": "hoali100@uni-duesseldorf.de",
"address": "Universit\u00e4tstr. 1, 40225 D\u00fcsseldorf",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "ORCID",
"value": "0009-0005-5539-497X"
}
]
},
{
"@id": "Tobias.Griguschies@uni-duesseldorf.de",
"firstName": "Tobias",
"lastName": "Griguschies",
"email": "Tobias.Griguschies@uni-duesseldorf.de",
"address": "Universit\u00e4tstr. 1, 40225 D\u00fcsseldorf",
"affiliation": "Heinrich-Heine-Universit\u00e4t"
},
{
"@id": "https://orcid.org/0000-0002-4383-6196",
"firstName": "Paloma",
"lastName": "Dur\u00e1n",
"email": "paloma.duran@inrae.fr",
"affiliation": "INRAE",
"comments": [
{
"name": "ORCID",
"value": "https://orcid.org/0000-0002-4383-6196"
}
]
},
{
"@id": "0000-0002-3872-3613",
"firstName": "Tobias",
"lastName": "Lautwein",
"email": "Tobias.Lautwein@hhu.de",
"address": "Universit\u00e4tstr. 1, 40225 D\u00fcsseldorf",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "ORCID",
"value": "0000-0002-3872-3613"
}
]
},
{
"@id": "s.metzger@uni-koeln.de",
"firstName": "Sabine",
"lastName": "Metzger",
"email": "s.metzger@uni-koeln.de",
"affiliation": "Universit\u00e4t zu K\u00f6ln"
},
{
"@id": "https://orcid.org/0000-0002-7622-1139",
"firstName": "Eliza",
"lastName": "Loo",
"midInitials": "PI",
"email": "loo@hhu.de",
"address": "Universit\u00e4tstr. 1, 40225 D\u00fcsseldorf",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "ORCID",
"value": "https://orcid.org/0000-0002-7622-1139"
}
]
}
],
"studies": [
{
"@id": "studies/Aqueous_Soil_Extracts_ASE/",
"filename": "studies/Aqueous Soil Extracts ASE/isa.study.xlsx",
"identifier": "Aqueous Soil Extracts ASE",
"title": "ArtSoil accommodates various aqueous soil extracts ",
"description": "To determine if ArtSoil is compatible with ASEs of other soils, four ASEs derived from native soils ArtSoilCAS, Reijerskamp (ArtSoilREI), Oranjewoud (ArtSoilORJ) and Aijen (ArtSoilAI); and two commercial potting soils (i.e. soil enriched with macronutrients), Bedding Plant substrate (ArtSoilBP, https://klasmann-deilmann.com/de/anwendungsfelder/beet-und-balkonpflanzen) and D\u00fcsovit (ArtSoilDUS, https://www.duesovit.de/blumenerde.htm), were tested",
"people": [
{
"@id": "https://orcid.org/0000-0002-7622-1139",
"firstName": "Eliza",
"lastName": "Loo",
"midInitials": "PI",
"email": "loo@hhu.de",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "performer (ARC:00000168) Microbiota Profiling",
"value": "Microbiota Profiling"
},
{
"name": "ORCID",
"value": "https://orcid.org/0000-0002-7622-1139"
}
]
},
{
"@id": "Tobias.Lautwein@hhu.de",
"firstName": "Tobias",
"lastName": "Lautwein",
"email": "Tobias.Lautwein@hhu.de",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "performer (ARC:00000168) Shoot Fresh Mass",
"value": "Shoot Fresh Mass"
}
]
},
{
"@id": "hoali100@uni-duesseldorf.de",
"firstName": "Houda",
"lastName": "Alioui",
"email": "hoali100@uni-duesseldorf.de",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "performer (ARC:00000168) Shoot Fresh Mass",
"value": "Shoot Fresh Mass"
}
]
},
{
"@id": "https://orcid.org/0000-0002-7622-1139",
"firstName": "Eliza",
"lastName": "Loo",
"midInitials": "PI",
"email": "loo@hhu.de",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "performer (ARC:00000168) Shoot Fresh Mass",
"value": "Shoot Fresh Mass"
},
{
"name": "ORCID",
"value": "https://orcid.org/0000-0002-7622-1139"
}
]
},
{
"@id": "Tobias.Lautwein@hhu.de",
"firstName": "Tobias",
"lastName": "Lautwein",
"email": "Tobias.Lautwein@hhu.de",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "performer (ARC:00000168) Single-cell Transcriptomics",
"value": "Single-cell Transcriptomics"
}
]
},
{
"@id": "Vera.Kaplunova@hhu.de",
"firstName": "Vera",
"lastName": "Kaplunova",
"email": "Vera.Kaplunova@hhu.de",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "performer (ARC:00000168) Single-cell Transcriptomics",
"value": "Single-cell Transcriptomics"
}
]
},
{
"@id": "https://orcid.org/0000-0002-7622-1139",
"firstName": "Eliza",
"lastName": "Loo",
"midInitials": "PI",
"email": "loo@hhu.de",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "performer (ARC:00000168) Single-cell Transcriptomics",
"value": "Single-cell Transcriptomics"
},
{
"name": "ORCID",
"value": "https://orcid.org/0000-0002-7622-1139"
}
]
},
{
"@id": "s.metzger@uni-koeln.de",
"firstName": "Sabine",
"lastName": "Metzger",
"email": "s.metzger@uni-koeln.de",
"affiliation": "Universit\u00e4t zu K\u00f6ln",
"comments": [
{
"name": "performer (ARC:00000168) Soil Elemental Analysis",
"value": "Soil Elemental Analysis"
}
]
},
{
"@id": "hoali100@uni-duesseldorf.de",
"firstName": "Houda",
"lastName": "Alioui",
"email": "hoali100@uni-duesseldorf.de",
"affiliation": "Heinrich-Heine-Universit\u00e4t",
"comments": [
{
"name": "performer (ARC:00000168) Total Root Length",
"value": "Total Root Length"
}
]
}
],
"protocols": [
{
"@id": "#Protocol_./assays/Shoot_Fresh_Mass/protocols/Shoot_fresh_mass_measurement.md",
"name": "./assays/Shoot Fresh Mass/protocols/Shoot fresh mass measurement.md"
},
{
"@id": "#Protocol_./assays/Total_Root_Length/protocols",
"name": "./assays/Total Root Length/protocols"
},
{
"@id": "#Protocol_./assays/Soil_Elemental_Analysis/protocols/ICP-MS_analysis.md",
"name": "./assays/Soil Elemental Analysis/protocols/ICP-MS analysis.md"
},
{
"@id": "#Protocol_./assays/Microbiota_Profiling/protocols/16S_amplicon_sequencing.md",
"name": "./assays/Microbiota Profiling/protocols/16S amplicon sequencing.md"
},
{
"@id": "#Protocol_./assays/Single-cell_Transcriptomics/protocols/Protoplast_isolation_and_sequencing.md",
"name": "./assays/Single-cell Transcriptomics/protocols/Protoplast isolation and sequencing.md"
}
],
"materials": {
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"@id": "#Sample_Aijen",
"name": "Aijen"
},
{
"@id": "#Sample_BP",
"name": "BP"
},
{
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"name": "CAS"
},
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"name": "DUS"
},
{
"@id": "#Sample_Oranje",
"name": "Oranje"
},
{
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"name": "REI"
},
{
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"factorType": {
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},
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"name": "Metadata for ArtSoil study_0",
"executesProtocol": {
"@id": "#Protocol_./assays/Shoot_Fresh_Mass/protocols/Shoot_fresh_mass_measurement.md",
"name": "./assays/Shoot Fresh Mass/protocols/Shoot fresh mass measurement.md"
},
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{
"@id": "#Sample_Aijen",
"name": "Aijen"
},
{
"@id": "#Sample_BP",
"name": "BP"
},
{
"@id": "#Sample_CAS",
"name": "CAS"
},
{
"@id": "#Sample_DUS",
"name": "DUS"
},
{
"@id": "#Sample_Oranje",
"name": "Oranje"
},
{
"@id": "#Sample_REI",
"name": "REI"
}
],
"outputs": [
{
"@id": "#Sample_ArtSoilAIJ",
"name": "ArtSoilAIJ",
"factorValues": [
{
"@id": "#FactorValue/Plant=Arabidopsis thaliana",
"category": {
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}
},
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"annotationValue": "Arabidopsis thaliana",
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}
},
{
"@id": "#FactorValue/Tissue=Root",
"category": {
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"factorName": "Factor [Tissue]",
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},
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}
},
{
"@id": "#FactorValue/agricultural soil=Aijen",
"category": {
"@id": "#Factor/Factor [agricultural soil]",
"factorName": "Factor [agricultural soil]",
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},
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"@id": "#UserTerm_Aijen",
"annotationValue": "Aijen"
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}
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},
{
"@id": "#Sample_ArtSoilBP",
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},
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},
{
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},
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},
{
"@id": "#FactorValue/agricultural soil=BP-substrate",
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"factorName": "Factor [agricultural soil]",
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},
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"@id": "#UserTerm_BP-substrate",
"annotationValue": "BP-substrate"
}
}
]
},
{
"@id": "#Sample_ArtSoilCAS",
"name": "ArtSoilCAS",
"factorValues": [
{
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},
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"annotationValue": "Arabidopsis thaliana",
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"termAccession": "http://purl.obolibrary.org/obo/C_33292"
}
},
{
"@id": "#FactorValue/Tissue=Root",
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}
},
{
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}
},
"value": {
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"annotationValue": "CAS"
}
}
]
},
{
"@id": "#Sample_ArtSoilDUS",
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}
},
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"annotationValue": "Arabidopsis thaliana",
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}
},
{
"@id": "#FactorValue/Tissue=Root",
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},
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},
{
"@id": "#FactorValue/agricultural soil=D\u00fcsovit",
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},
"value": {
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"annotationValue": "D\u00fcsovit"
}
}
]
},
{
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},
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},
{
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},
{
"@id": "#FactorValue/agricultural soil=Oranjewoud",
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}
},
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{
"@id": "#Sample_ArtSoilREI",
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},
{
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{
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},
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"annotationValue": "Reijeskamp"
}
}
]
}
],
"comments": [
{
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"value": ""
}
]
},
{
"@id": "#Process_Metadata_for_ArtSoil_study_1",
"name": "Metadata for ArtSoil study_1",
"executesProtocol": {
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"name": "./assays/Total Root Length/protocols"
},
"inputs": [
{
"@id": "#Sample_CAS4",
"name": "CAS4"
},
{
"@id": "#Sample_MS",
"name": "MS"
},
{
"@id": "#Sample_SUC",
"name": "SUC"
}
],
"outputs": [
{
"@id": "#Sample_ArtSoilCAS",
"name": "ArtSoilCAS",
"factorValues": [
{
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"category": {
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}
},
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}
},
{
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}
},
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},
{
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},
"value": {
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}
]
},
{
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},
{
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}
},
{
"@id": "#FactorValue/agricultural soil=1/2MS media without sucrose",
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},
"value": {
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}
]
},
{
"@id": "#Sample_1/2MS+Suc",
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}
},
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}
},
{
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}
},
{
"@id": "#FactorValue/agricultural soil=1/2MS media with sucrose",
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},
"value": {
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"annotationValue": "1/2MS media with sucrose"
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}
]
}
],
"comments": [
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"value": ""
}
]
},
{
"@id": "#Process_Metadata_for_ArtSoil_study_2",
"name": "Metadata for ArtSoil study_2",
"executesProtocol": {
"@id": "#Protocol_./assays/Soil_Elemental_Analysis/protocols/ICP-MS_analysis.md",
"name": "./assays/Soil Elemental Analysis/protocols/ICP-MS analysis.md"
},
"inputs": [
{
"@id": "#Sample_Reijerscamp",
"name": "Reijerscamp"
},
{
"@id": "#Sample_Aijen",
"name": "Aijen"
},
{
"@id": "#Sample_Oranjewoud",
"name": "Oranjewoud"
},
{
"@id": "#Sample_BP-Substrate",
"name": "BP-Substrate"
},
{
"@id": "#Sample_CAS",
"name": "CAS"
},
{
"@id": "#Sample_D\u00fcsovit",
"name": "D\u00fcsovit"
}
],
"outputs": [
{
"@id": "#Sample_REI",
"name": "REI",
"factorValues": [
{
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"category": {
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},
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}
},
{
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}
},
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}
},
{
"@id": "#FactorValue/agricultural soil=Reijeskamp",
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}
},
"value": {
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}
}
]
},
{
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},
{
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},
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},
{
"@id": "#FactorValue/agricultural soil=Aijen",
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},
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]
},
{
"@id": "#Sample_ORJ",
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},
{
"@id": "#FactorValue/Tissue=Root",
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},
{
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}
},
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}
]
},
{
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"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_BP",
"name": "Soil type",
"value": "BP"
},
{
"@id": "#Comment_Biological_Replicate_2",
"name": "Biological Replicate",
"value": "2"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_6",
"name": "Metadata for SFW_6",
"executesProtocol": {
"@id": "#Protocol_./assays/Shoot_Fresh_Mass/protocols/Shoot_fresh_mass_measurement.md",
"name": "./assays/Shoot Fresh Mass/protocols/Shoot fresh mass measurement.md",
"parameters": [
{
"@id": "#ProtocolParameter/PATO:0000011",
"parameterName": {
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"annotationValue": "age",
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}
}
],
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{
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"termSource": "C",
"termAccession": "C:3395"
}
}
]
},
"parameterValues": [
{
"category": {
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"parameterName": {
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}
},
"value": 4,
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"annotationValue": "week",
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"termAccession": "https://bioregistry.io/UO:0000034"
}
}
],
"inputs": [
{
"@id": "#Source_ArtSoilBP",
"name": "ArtSoilBP"
},
{
"@id": "#Source_ArtSoilBP",
"name": "ArtSoilBP"
}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_BP",
"name": "Soil type",
"value": "BP"
},
{
"@id": "#Comment_Biological_Replicate_3",
"name": "Biological Replicate",
"value": "3"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_7",
"name": "Metadata for SFW_7",
"executesProtocol": {
"@id": "#Protocol_./assays/Shoot_Fresh_Mass/protocols/Shoot_fresh_mass_measurement.md",
"name": "./assays/Shoot Fresh Mass/protocols/Shoot fresh mass measurement.md",
"parameters": [
{
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}
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}
}
]
},
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},
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"annotationValue": "week",
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}
],
"inputs": [
{
"@id": "#Source_ArtSoilBP",
"name": "ArtSoilBP"
},
{
"@id": "#Source_ArtSoilBP",
"name": "ArtSoilBP"
}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_BP",
"name": "Soil type",
"value": "BP"
},
{
"@id": "#Comment_Biological_Replicate_4",
"name": "Biological Replicate",
"value": "4"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_8",
"name": "Metadata for SFW_8",
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"name": "./assays/Shoot Fresh Mass/protocols/Shoot fresh mass measurement.md",
"parameters": [
{
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}
}
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},
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},
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}
],
"inputs": [
{
"@id": "#Source_ArtSoilCAS",
"name": "ArtSoilCAS"
},
{
"@id": "#Source_ArtSoilCAS",
"name": "ArtSoilCAS"
}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_CAS",
"name": "Soil type",
"value": "CAS"
},
{
"@id": "#Comment_Biological_Replicate_1",
"name": "Biological Replicate",
"value": "1"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_9",
"name": "Metadata for SFW_9",
"executesProtocol": {
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"parameters": [
{
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],
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}
]
},
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}
],
"inputs": [
{
"@id": "#Source_ArtSoilCAS",
"name": "ArtSoilCAS"
},
{
"@id": "#Source_ArtSoilCAS",
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}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_CAS",
"name": "Soil type",
"value": "CAS"
},
{
"@id": "#Comment_Biological_Replicate_2",
"name": "Biological Replicate",
"value": "2"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_10",
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}
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},
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],
"inputs": [
{
"@id": "#Source_ArtSoilCAS",
"name": "ArtSoilCAS"
},
{
"@id": "#Source_ArtSoilCAS",
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}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_CAS",
"name": "Soil type",
"value": "CAS"
},
{
"@id": "#Comment_Biological_Replicate_3",
"name": "Biological Replicate",
"value": "3"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_11",
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],
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"name": "ArtSoilCAS"
},
{
"@id": "#Source_ArtSoilCAS",
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],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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},
{
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}
],
"comments": [
{
"@id": "#Comment_Soil_type_CAS",
"name": "Soil type",
"value": "CAS"
},
{
"@id": "#Comment_Biological_Replicate_4",
"name": "Biological Replicate",
"value": "4"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_12",
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],
"inputs": [
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"name": "ArtSoilAIJ"
},
{
"@id": "#Source_ArtSoilAIJ",
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}
],
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{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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},
{
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}
],
"comments": [
{
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"name": "Soil type",
"value": "Aijen"
},
{
"@id": "#Comment_Biological_Replicate_1",
"name": "Biological Replicate",
"value": "1"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_13",
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],
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"name": "ArtSoilAIJ"
},
{
"@id": "#Source_ArtSoilAIJ",
"name": "ArtSoilAIJ"
}
],
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{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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},
{
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"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_Aijen",
"name": "Soil type",
"value": "Aijen"
},
{
"@id": "#Comment_Biological_Replicate_2",
"name": "Biological Replicate",
"value": "2"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_14",
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}
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}
],
"inputs": [
{
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],
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{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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}
],
"comments": [
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},
{
"@id": "#Comment_Biological_Replicate_3",
"name": "Biological Replicate",
"value": "3"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_15",
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},
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}
],
"inputs": [
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}
],
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{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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{
"@id": "#Comment_Biological_Replicate_4",
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]
},
{
"@id": "#Process_Metadata_for_SFW_16",
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}
},
"value": 4,
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"annotationValue": "week",
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}
}
],
"inputs": [
{
"@id": "#Source_ArtSoilDUS",
"name": "ArtSoilDUS"
},
{
"@id": "#Source_ArtSoilDUS",
"name": "ArtSoilDUS"
}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_Dus",
"name": "Soil type",
"value": "Dus"
},
{
"@id": "#Comment_Biological_Replicate_1",
"name": "Biological Replicate",
"value": "1"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_17",
"name": "Metadata for SFW_17",
"executesProtocol": {
"@id": "#Protocol_./assays/Shoot_Fresh_Mass/protocols/Shoot_fresh_mass_measurement.md",
"name": "./assays/Shoot Fresh Mass/protocols/Shoot fresh mass measurement.md",
"parameters": [
{
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"parameterName": {
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}
}
],
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}
}
]
},
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{
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}
},
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],
"inputs": [
{
"@id": "#Source_ArtSoilDUS",
"name": "ArtSoilDUS"
},
{
"@id": "#Source_ArtSoilDUS",
"name": "ArtSoilDUS"
}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_Dus",
"name": "Soil type",
"value": "Dus"
},
{
"@id": "#Comment_Biological_Replicate_2",
"name": "Biological Replicate",
"value": "2"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_18",
"name": "Metadata for SFW_18",
"executesProtocol": {
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"parameters": [
{
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}
}
]
},
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],
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"name": "ArtSoilDUS"
},
{
"@id": "#Source_ArtSoilDUS",
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}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
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"name": "Soil type",
"value": "Dus"
},
{
"@id": "#Comment_Biological_Replicate_3",
"name": "Biological Replicate",
"value": "3"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_19",
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"executesProtocol": {
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"parameters": [
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}
]
},
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],
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"name": "ArtSoilDUS"
},
{
"@id": "#Source_ArtSoilDUS",
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}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
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"name": "Soil type",
"value": "Dus"
},
{
"@id": "#Comment_Biological_Replicate_4",
"name": "Biological Replicate",
"value": "4"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_20",
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{
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}
]
},
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],
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"@id": "#Source_ArtSoilREI",
"name": "ArtSoilREI"
},
{
"@id": "#Source_ArtSoilREI",
"name": "ArtSoilREI"
}
],
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{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
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"value": "REI"
},
{
"@id": "#Comment_Biological_Replicate_1",
"name": "Biological Replicate",
"value": "1"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_21",
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],
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"name": "ArtSoilREI"
},
{
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}
],
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"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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},
{
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}
],
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"value": "REI"
},
{
"@id": "#Comment_Biological_Replicate_2",
"name": "Biological Replicate",
"value": "2"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_22",
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],
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},
{
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}
],
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"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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},
{
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"type": "Raw Data File"
}
],
"comments": [
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"value": "REI"
},
{
"@id": "#Comment_Biological_Replicate_3",
"name": "Biological Replicate",
"value": "3"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_23",
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},
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],
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"name": "ArtSoilREI"
},
{
"@id": "#Source_ArtSoilREI",
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}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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"type": "Raw Data File"
}
],
"comments": [
{
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"name": "Soil type",
"value": "REI"
},
{
"@id": "#Comment_Biological_Replicate_4",
"name": "Biological Replicate",
"value": "4"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_24",
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],
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}
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},
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],
"inputs": [
{
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"name": "1/2MS-Suc"
},
{
"@id": "#Source_1/2MS-Suc",
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}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_MS",
"name": "Soil type",
"value": "MS"
},
{
"@id": "#Comment_Biological_Replicate_1",
"name": "Biological Replicate",
"value": "1"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_25",
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"parameters": [
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],
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}
]
},
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],
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"name": "1/2MS-Suc"
},
{
"@id": "#Source_1/2MS-Suc",
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}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
"comments": [
{
"@id": "#Comment_Soil_type_MS",
"name": "Soil type",
"value": "MS"
},
{
"@id": "#Comment_Biological_Replicate_2",
"name": "Biological Replicate",
"value": "2"
}
]
},
{
"@id": "#Process_Metadata_for_SFW_26",
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"executesProtocol": {
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"parameters": [
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],
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}
]
},
"parameterValues": [
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},
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],
"inputs": [
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"name": "1/2MS-Suc"
},
{
"@id": "#Source_1/2MS-Suc",
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}
],
"outputs": [
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
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},
{
"@id": "./assays/Shoot_Fresh_Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"name": "./assays/Shoot Fresh Mass/dataset/SFWArtSoilAllASEOpen.xlsx",
"type": "Raw Data File"
}
],
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{
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},
{
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{
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{
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{
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{
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{
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{
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{
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{
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{
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