[Data set] In vivo online monitoring of intracellular lipid accumulation in Ustilago maydis

Registered ARC content:

• root
  • README.md
  • isa.investigation.xlsx
  • assays
    • A1_Western blot analysis LD reporter candidates
      • README.md
      • isa.assay.xlsx
      • dataset
        • Rawdata
          • 20250116_WesternVIIII_Ux53-55_GFP_increment_30s 20250117_015012-07_Ch_Chemi+Marker.jpg
          • 20250121_UX53-55_Aktin+GFP_2min 20250122_023040_Ch_Chemi+Marker.jpg
    • A2_Protein localization LD reporter candidates
      • README.md
      • isa.assay.xlsx
      • dataset
        • Rawdata
          • Data_Erg6-Gfp_microscopy
            • - Day1_N limitation_Ux54_BF-DIC-1.tif - Day1_N limitation_Ux54_Laser488-1.tif - Day2_N limitation_UX54_BF-DIC-2.tif - Day2_N limitation_UX54_Laser488-2.tif
          • Data_Per1-Gfp_microscopy
            • - Day1_N limitation_Ux53_BF-DIC-2.tif - Day1_N limitation_Ux53_Laser488-2.tif - Day3_N limitation_UX53_BF-DIC.tif - Day3_N limitation_UX53_Laser488.tif
          • Data_Sei1-Gfp_microscopy
            • - Day1_N limitation_Ux55_BF-DIC-1.tif - Day1_N limitation_Ux55_Laser488-1.tif - Day2_N limitation_UX55_BF-DIC-3.tif - Day2_N limitation_UX55_Laser488-3.tif
          • Data_WT_microscopy
            • - Day1_N limitation_UMa2112_BF-DIC-1.tif - Day1_N limitation_UMa2112_Laser488-1.tif - Day3_N limitation_UMa2112_BF-DIC-3.tif - Day3_N limitation_UMa2112_Laser488-3.tif
    • A3_Erg6 protein localization with different promotors
      • README.md
      • isa.assay.xlsx
      • dataset
        • Rawdata
          • Data_Pcyp1 Erg6-Gfp_microscopy
            • - Day1_N limitation_Ux81_BF-DIC-1.tif - Day1_N limitation_Ux81_Laser488-1.tif - Day2_N limitation_Ux81_BF-DIC-1.tif - Day2_N limitation_Ux81_Laser488-1.tif - Day3_N limitation_Ux81_BF-DIC-1.tif - Day3_N limitation_Ux81_Laser488-1.tif
          • Data_Pcyp2 Erg6-Gfp_microscopy
            • - Day1_N limitation_Ux82_BF-DIC.tif - Day1_N limitation_Ux82_Laser488.tif - Day2_N limitation_Ux82_BF-DIC-6.tif - Day2_N limitation_Ux82_Laser488-6.tif - Day3_N limitation_Ux82_BF-DIC-3.tif - Day3_N limitation_Ux82_Laser488-3.tif
          • Data_Pemt1 Erg6-Gfp_microscopy
            • - Day1_N limitation_Ux83_BF-DIC-2.tif - Day1_N limitation_Ux83_Laser488-2.tif - Day2_N limitation_Ux83_BF-DIC-6.tif - Day2_N limitation_Ux83_Laser488-6.tif - Day3_N limitation_Ux83_BF-DIC-2.tif - Day3_N limitation_Ux83_Laser488-2.tif
          • Data_WT_microscopy2
            • - Day1_N limitation_UMa2112_BF-DIC-1.tif - Day1_N limitation_UMa2112_Laser488-1.tif - Day2_N limitation_UMa2112_BF-DIC-1.tif - Day2_N limitation_UMa2112_Laser488-1.tif - Day3_N limitation_UMa2112_BF-DIC-2.tif - Day3_N limitation_UMa2112_Laser488-2.tif
    • A4_Erg6-Kat protein localization
      • README.md
      • isa.assay.xlsx
      • dataset
        • Rawdata
          • Data_Erg6-Kat2x_microscopy
            • - Day1_N limitation_Ux143_2x_BF-DIC-5.tif - Day1_N limitation_Ux143_2x_Laser488-5.tif - Day1_N limitation_Ux143_2x_Laser561-5.tif - Day2_N limitation_UX143_2x_BF-DIC-8.tif - Day2_N limitation_UX143_2x_Laser488-10.tif - Day2_N limitation_UX143_2x_Laser561-4.tif - Day3_N limitation_UX143_2x_BF-DIC-5.tif - Day3_N limitation_UX143_2x_Laser488-5.tif - Day3_N limitation_UX143_2x_Laser561-5.tif
          • Data_Erg6-Kat_microscopy
            • - Day1_N limitation_UX143_1x_BF-DIC.tif - Day1_N limitation_UX143_1x_Laser488.tif - Day1_N limitation_UX143_1x_Laser561.tif - Day2_N limitation_UX143_1x_BF-DIC-1.tif - Day2_N limitation_UX143_1x_Laser488-1.tif - Day2_N limitation_UX143_1x_Laser561-1.tif - Day3_N limitation_UX143_1x_BF-DIC-3.tif - Day3_N limitation_UX143_1x_Laser488-3.tif - Day3_N limitation_UX143_1x_Laser561-3.tif
          • Data_Erg6-Katmx_microscopy
            • - Day1_N limitation_UX143_3x_BF-DIC-3.tif - Day1_N limitation_UX143_3x_Laser488-3.tif - Day1_N limitation_UX143_3x_Laser561-3.tif - Day2_N limitation_UX143_3x_BF-DIC-5.tif - Day2_N limitation_UX143_3x_Laser488-5.tif - Day2_N limitation_UX143_3x_Laser561-5.tif - Day3_N limitation_UX143_3x_BF-DIC-4.tif - Day3_N limitation_UX143_3x_Laser488-4.tif - Day3_N limitation_UX143_3x_Laser561-4.tif
          • Data_WT_microscopy3
            • - Day1_N limitation_UMa2112_BF-DIC.tif - Day1_N limitation_UMa2112_Laser488.tif - Day2_N limitation_UMa2112_BF-DIC.tif - Day2_N limitation_UMa2112_Laser488.tif - Day3_N limitation_UMa2112_BF-DIC-3.tif - Day3_N limitation_UMa2112_Laser488-3.tif - Day3_N limitation_UMa2112_Laser561-3.tif
    • A5_Quantification of LD numbers in Erg6-Kat overexpression
      • README.md
      • isa.assay.xlsx
      • dataset
        • Rawdata
          • LD_quantification_raw.xlsx
          • Erg6-Kat2x_pictures_quantif
            • - Day3_N limitation_UX143_2x_BF-DIC-1.tif
          • Erg6-Kat_pictures_quantif
            • - Day3_N limitation_UX143_1x_BF-DIC-3.tif
          • Erg6-Katmx_pictures_quantif
            • - Day3_N limitation_UX143_3x_BF-DIC-4.tif
          • WT_picutures_quantif
            • - Day3_N limitation_UMa2112_BF-DIC-2.tif
    • A6_Biolector growth monitoring LD accumulation
      • README.md
      • isa.assay.xlsx
      • dataset
        • Rawdata
          • BioLector_run1
            • - Biolector dataset_1.pzfx - Replicate_1_2007-0500_20231130_TH002_20231130_163626_N1_13-34-16.xlsx
          • BioLector_run2
            • - Biolector dataset_5.pzfx - Replicate 5_2007-0500_20240815-TH003_20060101_140757_N1_07-10-24.xlsx
          • BioLector_run3
            • - Biolector dataset_6.pzfx - Replicate 6_2007-0500_20241010-TH006_20060101_145525_N1_12-16-13.xlsx
  • studies
    • S1_Identification of a LD reporter candidate
      • README.md
      • isa.study.xlsx
    • S2_Erg6 biosensor optimization
      • README.md
      • isa.study.xlsx
    • S3_In vivo monitoring with the optimized biosensor Erg6-Kat2x
      • README.md
      • isa.study.xlsx