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"@id": "#CharacteristicValue_strain_collection_number_UMa2112",
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"additionalType": "CharacteristicValue",
"name": "strain collection number",
"value": "UMa2112",
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},
{
"@id": "#CharacteristicValue_Genotype_MB215\u0394cyp1\u0394emt1\t\t",
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"additionalType": "CharacteristicValue",
"name": "Genotype",
"value": "MB215\u0394cyp1\u0394emt1\t\t",
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},
{
"@id": "#Sample_UMa2112",
"@type": "Sample",
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"name": "UMa2112",
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{
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},
{
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},
{
"@id": "#CharacteristicValue_DNA_Sequence_./studies/S1_Identification_of_a_LD_reporter_candidate/resources/Strain_sequences_modified_locus"
},
{
"@id": "#CharacteristicValue_Genotype_MB215\u0394cyp1\u0394emt1"
},
{
"@id": "#CharacteristicValue_expression_unit_copy_number"
}
]
},
{
"@id": "#Process_S_S1_Identification_of_a_LD_reporter_candidate_Strains_1",
"@type": "LabProcess",
"name": "Strains_1",
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},
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},
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}
},
{
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},
{
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},
{
"@id": "#Sample_Per1-Gfp",
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},
{
"@id": "#Process_S_S1_Identification_of_a_LD_reporter_candidate_Strains_2",
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}
},
{
"@id": "#CharacteristicValue_Genotype_MB215\u0394cyp1\u0394emt1",
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},
{
"@id": "#Process_S_S1_Identification_of_a_LD_reporter_candidate_Strains_3",
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},
{
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{
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},
{
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},
{
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},
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}
},
{
"@id": "#Process_S_S1_Identification_of_a_LD_reporter_candidate_Strains_5",
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},
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}
},
{
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}
},
{
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}
},
{
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]
},
{
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},
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{
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},
{
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]
},
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"propertyID": "http://purl.org/nfdi4plants/ontology/dpbo/DPBO_1000155",
"unitCode": "https://bioregistry.io/UO:0000098",
"unitText": "milliliter",
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},
{
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},
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{
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{
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},
{
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},
{
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}
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{
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{
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},
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"unitText": "Revolution per Minute",
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},
{
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{
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},
{
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},
{
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}
]
},
{
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"name": "Sei1-Gfp_preculture",
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}
},
{
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},
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},
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},
{
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},
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},
{
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},
{
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}
]
},
{
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}
},
{
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},
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{
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},
{
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]
},
{
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},
{
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},
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},
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{
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},
{
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},
{
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},
{
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{
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]
},
{
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"additionalType": "Study",
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"dateModified": "2025-10-09T11:06:23.8413888",
"description": "3 candidates for potential lipid droplet biosensors were selected (sei1, per1, erg6). Three strains were generated that express the respective genes fused to a sequence for the HA tag under control of the mmt1 promoter that is induced upon nitrogen starvation, i.e. when enhanced lipid droplet production is induced. Strains were tested by Western blot analyses for synthesis of the proteins using anti-HA antibodies. The localisation and expression was verified using fluorescence microscopy. ",
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{
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},
{
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},
{
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},
{
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},
{
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},
{
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},
{
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}
],
"name": "Identification of a LD reporter candidate",
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},
{
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},
{
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},
{
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},
{
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},
{
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{
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},
{
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},
{
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},
{
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},
{
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},
{
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{
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{
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},
{
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