{
"@type": "CreativeWork",
"@id": "ro-crate-metadata.json",
"about": {
"@id": "./",
"@type": "Investigation",
"additionalType": "Investigation",
"identifier": "2025_Expression_System_Adeanei",
"filename": "isa.investigation.xlsx",
"title": "T7 RNA polymerase-based gene expression from a transcriptionally silent rDNA spacer in the endosymbiont-harboring trypanosomatid Angomonas deanei",
"description": "Eukaryotic life has been shaped fundamentally by the integration of bacterial endosymbionts. The trypanosomatid Angomonas deanei that contains a beta-proteobacterial endosymbiont, represents an emerging model to elucidate initial steps in symbiont integration. Although the repertoire of genetic tools for A. deanei is growing, no conditional gene expression system is available yet, which would be key for the functional characterization of essential or expression of toxic proteins. Development of a conditional expression system based on endogenous RNA polymerase II (POLII) is hampered by the absence of information on transcription signals in A. deanei as well as the unusual genetic system used in the Trypanosomatidae that relies on read-through transcription. This mode of transcription can result in polar effects when manipulating expression of genes in their endogenous loci. Finally, only few resistance markers are available for A. deanei yet, restricting the number of genetic modifications that can be introduced into one strain. To increase the range of possible genetic manipulations in A. deanei, and in particular, build the base for a conditional expression system that does not interfere with the endogenous gene expression machinery, here we (i) implemented two new drug resistance markers, (ii) identified the spacer upstream of the rDNA array on chromosome 13 as transcriptionally silent genomic locus, and (iii) used this locus for engineering an ectopic expression system that depends on the T7 RNA polymerase expressed from the delta-amastin locus. We show that transgene expression in this system is independent of the activity of endogenous RNA polymerases, reaches expression levels similar to the previously described POLII-dependent expression from the gamma-amastin locus, and can be applied for studying endosymbiosis. In sum, the new tools expand the possibilities for genetic manipulations of A. deanei and provide a solid base for the development of an ectopic conditional expression system.",
"people": [
{
"@id": "lena.kroeninger@hhu.de",
"@type": "Person",
"firstName": "Lena",
"lastName": "Kr\u00f6ninger",
"email": "lena.kroeninger@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "anay.maurya@hhu.de",
"@type": "Person",
"firstName": "Anay Kumar",
"lastName": "Maurya",
"email": "anay.maurya@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "christian.stiebeling@hhu.de",
"@type": "Person",
"firstName": "Christian",
"lastName": "Stiebeling",
"email": "christian.stiebeling@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "florian..stirba@hhu.de",
"@type": "Person",
"firstName": "Florian",
"lastName": "Stirba",
"email": "florian..stirba@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "zio.kim@hhu.de",
"@type": "Person",
"firstName": "Zio",
"lastName": "Kim",
"email": "zio.kim@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "e.nowack@hhu.de",
"@type": "Person",
"firstName": "Eva",
"lastName": "Nowack",
"email": "e.nowack@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000380",
"@type": "OntologyAnnotation",
"annotationValue": "principal investigator",
"termSource": "AGRO",
"termAccession": "AGRO:00000380",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"studies": [
{
"@id": "#study/S1_Identification-of-potentially-silent-loci",
"@type": [
"Study"
],
"additionalType": "Study",
"identifier": "S1_Identification-of-potentially-silent-loci",
"filename": "studies/S1_Identification-of-potentially-silent-loci/isa.study.xlsx",
"title": "Identification of potentially silent loci",
"submissionDate": "2025-01-30T00:29",
"people": [
{
"@id": "lena.kroeninger@hhu.de",
"@type": "Person",
"firstName": "Lena",
"lastName": "Kr\u00f6ninger",
"email": "lena.kroeninger@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "e.nowack@hhu.de",
"@type": "Person",
"firstName": "Eva",
"lastName": "Nowack",
"email": "e.nowack@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000380",
"@type": "OntologyAnnotation",
"annotationValue": "principal investigator",
"termSource": "AGRO",
"termAccession": "AGRO:00000380",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Study": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"assays": "sdo:hasPart",
"filename": "sdo:alternateName",
"comments": "sdo:comment",
"processSequence": "sdo:about",
"studyDesignDescriptors": "arc:ARC#ARC_00000037"
}
},
{
"@id": "#study/S5_Analysis-of-gene-expression-strength",
"@type": [
"Study"
],
"additionalType": "Study",
"identifier": "S5_Analysis-of-gene-expression-strength",
"filename": "studies/S5_Analysis-of-gene-expression-strength/isa.study.xlsx",
"title": "Analysis of gene expression strength",
"people": [
{
"@id": "lena.kroeninger@hhu.de",
"@type": "Person",
"firstName": "Lena",
"lastName": "Kr\u00f6ninger",
"email": "lena.kroeninger@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "anay.maurya@hhu.de",
"@type": "Person",
"firstName": "Anay Kumar",
"lastName": "Maurya",
"email": "anay.maurya@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "e.nowack@hhu.de",
"@type": "Person",
"firstName": "Eva",
"lastName": "Nowack",
"email": "e.nowack@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000380",
"@type": "OntologyAnnotation",
"annotationValue": "principal investigator",
"termSource": "AGRO",
"termAccession": "AGRO:00000380",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Study": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"assays": "sdo:hasPart",
"filename": "sdo:alternateName",
"comments": "sdo:comment",
"processSequence": "sdo:about",
"studyDesignDescriptors": "arc:ARC#ARC_00000037"
}
},
{
"@id": "#study/S3_Implementation-of-new-antibiotic-resistance-cassettes",
"@type": [
"Study"
],
"additionalType": "Study",
"identifier": "S3_Implementation-of-new-antibiotic-resistance-cassettes",
"filename": "studies/S3_Implementation-of-new-antibiotic-resistance-cassettes/isa.study.xlsx",
"title": "Implementation of new antibiotic resistance cassettes",
"people": [
{
"@id": "lena.kroeninger@hhu.de",
"@type": "Person",
"firstName": "Lena",
"lastName": "Kr\u00f6ninger",
"email": "lena.kroeninger@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "christian.stiebeling@hhu.de",
"@type": "Person",
"firstName": "Christian",
"lastName": "Stiebeling",
"email": "christian.stiebeling@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "florian..stirba@hhu.de",
"@type": "Person",
"firstName": "Florian",
"lastName": "Stirba",
"email": "florian..stirba@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "e.nowack@hhu.de",
"@type": "Person",
"firstName": "Eva",
"lastName": "Nowack",
"email": "e.nowack@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000380",
"@type": "OntologyAnnotation",
"annotationValue": "principal investigator",
"termSource": "AGRO",
"termAccession": "AGRO:00000380",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Study": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"assays": "sdo:hasPart",
"filename": "sdo:alternateName",
"comments": "sdo:comment",
"processSequence": "sdo:about",
"studyDesignDescriptors": "arc:ARC#ARC_00000037"
}
},
{
"@id": "#study/S4_T7RNAP-driven-expression-from-the-rDNA-spacer",
"@type": [
"Study"
],
"additionalType": "Study",
"identifier": "S4_T7RNAP-driven-expression-from-the-rDNA-spacer",
"filename": "studies/S4_T7RNAP-driven-expression-from-the-rDNA-spacer/isa.study.xlsx",
"title": "T7RNAP driven expression from the rDNA spacer",
"people": [
{
"@id": "lena.kroeninger@hhu.de",
"@type": "Person",
"firstName": "Lena",
"lastName": "Kr\u00f6ninger",
"email": "lena.kroeninger@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "zio.kim@hhu.de",
"@type": "Person",
"firstName": "Zio",
"lastName": "Kim",
"email": "zio.kim@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "e.nowack@hhu.de",
"@type": "Person",
"firstName": "Eva",
"lastName": "Nowack",
"email": "e.nowack@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000380",
"@type": "OntologyAnnotation",
"annotationValue": "principal investigator",
"termSource": "AGRO",
"termAccession": "AGRO:00000380",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Study": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"assays": "sdo:hasPart",
"filename": "sdo:alternateName",
"comments": "sdo:comment",
"processSequence": "sdo:about",
"studyDesignDescriptors": "arc:ARC#ARC_00000037"
}
},
{
"@id": "#study/S2_Heterologous-expression-of-the-T7RNAP",
"@type": [
"Study"
],
"additionalType": "Study",
"identifier": "S2_Heterologous-expression-of-the-T7RNAP",
"filename": "studies/S2_Heterologous-expression-of-the-T7RNAP/isa.study.xlsx",
"title": "Heterologous expression of the T7RNAP",
"people": [
{
"@id": "lena.kroeninger@hhu.de",
"@type": "Person",
"firstName": "Lena",
"lastName": "Kr\u00f6ninger",
"email": "lena.kroeninger@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "anay.maurya@hhu.de",
"@type": "Person",
"firstName": "Anay Kumar",
"lastName": "Maurya",
"email": "anay.maurya@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000373",
"@type": "OntologyAnnotation",
"annotationValue": "researcher",
"termSource": "AGRO",
"termAccession": "AGRO:00000373",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "e.nowack@hhu.de",
"@type": "Person",
"firstName": "Eva",
"lastName": "Nowack",
"email": "e.nowack@hhu.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Heinrich_Heine_University",
"name": "Heinrich Heine University",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"roles": [
{
"@id": "AGRO:00000380",
"@type": "OntologyAnnotation",
"annotationValue": "principal investigator",
"termSource": "AGRO",
"termAccession": "AGRO:00000380",
"@context": {
"sdo": "http://schema.org/",
"OntologyAnnotation": "sdo:DefinedTerm",
"annotationValue": "sdo:name",
"termSource": "sdo:inDefinedTermSet",
"termAccession": "sdo:termCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Study": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"assays": "sdo:hasPart",
"filename": "sdo:alternateName",
"comments": "sdo:comment",
"processSequence": "sdo:about",
"studyDesignDescriptors": "arc:ARC#ARC_00000037"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Investigation": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"studies": "sdo:hasPart",
"ontologySourceReferences": "sdo:mentions",
"comments": "sdo:comment",
"filename": "sdo:alternateName"
}
},
"conformsTo": {
"@id": "https://w3id.org/ro/crate/1.1"
},
"@context": {
"sdo": "http://schema.org/",
"arc": "http://purl.org/nfdi4plants/ontology/",
"CreativeWork": "sdo:CreativeWork",
"about": "sdo:about",
"conformsTo": "sdo:conformsTo"
}
}