{
"@type": "CreativeWork",
"@id": "ro-crate-metadata.json",
"about": {
"@id": "./",
"@type": "Investigation",
"additionalType": "Investigation",
"identifier": "2025_IronAndHemeRegulatoryNetworks",
"filename": "isa.investigation.xlsx",
"title": "Genome-wide analysis of DtxR and HrrA regulons reveals novel targets and a high level of interconnectivity between iron and heme regulatory networks in Corynebacterium glutamicum ",
"description": "Iron is vital for most organisms, serving as cofactor in enzymes, regulatory proteins, and respiratory cytochromes. In Corynebacterium glutamicum, iron and heme homeostasis are tightly interconnected and controlled by the global regulators DtxR and HrrA. While DtxR senses intracellular Fe2+, HrrSA is activated by heme. This study provides the first genome-wide analysis of DtxR and HrrA binding dynamics under varying iron and heme conditions using chromatin affinity purification and sequencing (ChAP-seq). We revealed 25 novel DtxR targets and 210 previously unrecognized HrrA targets. Among these, metH, encoding homocysteine methyltransferase, and xerC, encoding a tyrosine recombinase, were bound by DtxR exclusively under heme conditions, underscoring condition-dependent variation. Activation of metH by DtxR links iron metabolism to methionine synthesis, potentially relevant for the mitigation of oxidative stress. Beyond novel targets, 16 shared targets between DtxR and HrrA, some with overlapping operator sequences, highlight their interconnected regulons. Strikingly, we demonstrate the significance of weak ChAP-Seq peaks that are often disregarded in global approaches, but feature an impact of the regulator on differential gene expression. These findings emphasize the importance of genome-wide profiling under different conditions to uncover novel targets and shed light on the complexity and dynamic nature of bacterial regulatory networks. ",
"people": [
{
"@id": "ai.krueger@fz-juelich.de",
"@type": "Person",
"orcid": "0000-0002-4172-2398",
"firstName": "Aileen",
"lastName": "Kr\u00fcger",
"email": "ai.krueger@fz-juelich.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_IBG-1:_Institute_of_Bio-_and_Geosciences,_Forschungszentrum_J\u00fclich_GmbH",
"name": "IBG-1: Institute of Bio- and Geosciences, Forschungszentrum J\u00fclich GmbH",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "j.frunzke@fz-juelich.de",
"@type": "Person",
"orcid": "0000-0001-6209-7950",
"firstName": "Julia",
"lastName": "Frunzke",
"email": "j.frunzke@fz-juelich.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_IBG-1:_Institute_of_Bio-_and_Geosciences,_Forschungszentrum_J\u00fclich_GmbH",
"name": "IBG-1: Institute of Bio- and Geosciences, Forschungszentrum J\u00fclich GmbH",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "ul.weber@fz-juelich.de",
"@type": "Person",
"firstName": "Ulrike",
"lastName": "Weber",
"email": "ul.weber@fz-juelich.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_IBG-1:_Institute_of_Bio-_and_Geosciences,_Forschungszentrum_J\u00fclich_GmbH",
"name": "IBG-1: Institute of Bio- and Geosciences, Forschungszentrum J\u00fclich GmbH",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"studies": [
{
"@id": "#study/3_ReporterStrainGeneration",
"@type": [
"Study"
],
"additionalType": "Study",
"identifier": "3_ReporterStrainGeneration",
"filename": "studies/3_ReporterStrainGeneration/isa.study.xlsx",
"title": "Reporter Assay Strain Generation",
"description": "In order to evaluate on ChAP-Seq targets, it is required to couple it to expression data. To investigate on the influence of DtxR on the respective new targets (where expression data for these targets was not evaluable from former microarrays (Wennerhold et al. 2006, Brune et al. 2006) as well as being able to check this at different conditions and deletion mutants, we want to perform reporter assays on selected genes. The overall idea about choosing genes was to choose each strong, middle and weak binding peaks for each the iron excess condition and the heme condition.",
"people": [
{
"@id": "ul.weber@fz-juelich.de",
"@type": "Person",
"firstName": "Ulrike",
"lastName": "Weber",
"email": "ul.weber@fz-juelich.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Forschungszentrum_J\u00fclich_GmbH",
"name": "Forschungszentrum J\u00fclich GmbH",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "ai.krueger@fz-juelich.de",
"@type": "Person",
"orcid": "0000-0001-7060-4879",
"firstName": "Aileen",
"lastName": "Kr\u00fcger",
"email": "ai.krueger@fz-juelich.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Forschungszentrum_J\u00fclich_GmbH",
"name": "Forschungszentrum J\u00fclich GmbH",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"processSequence": [
{
"@id": "#Process_ReporterPlasmidCloning_0",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_0",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=NCgl1781",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "NCgl1781",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_0",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_0",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCCTCCAATACAAGCGCTGCTACC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCCTCCAATACAAGCGCTGCTACC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCATGCAGGGGCTGGGGTATCAG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCATGCAGGGGCTGGGGTATCAG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-PNCgl1781-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl1781-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_1",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_1",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=xerC",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "xerC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_1",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_1",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCGAGTATCGCTACCGAAGCCG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCGAGTATCGCTACCGAAGCCG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCAACGCGCCACCTTCTTTTTTGACTC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCAACGCGCCACCTTCTTTTTTGACTC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-PxerC-venus",
"@type": [
"Sample"
],
"name": "pJC1-PxerC-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_2",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_2",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=qor3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "qor3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_2",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_2",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCCTATGTGCCTGCGCCACATTCTG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCCTATGTGCCTGCGCCACATTCTG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCAGGGATCAGGGGTATCCATTTGC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCAGGGATCAGGGGTATCCATTTGC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-Pqor3-venus",
"@type": [
"Sample"
],
"name": "pJC1-Pqor3-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_3",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_3",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=cepA",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "cepA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_3",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_3",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCGGCTACCAGCTGAGTTAGATTGATAAC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCGGCTACCAGCTGAGTTAGATTGATAAC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCACCGTGCTTCGGGCGCGGT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCACCGTGCTTCGGGCGCGGT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-PcepA-venus",
"@type": [
"Sample"
],
"name": "pJC1-PcepA-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_4",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_4",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=NCgl2766",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "NCgl2766",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_4",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_4",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCCACATCCCCACGTTACCCCA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCCACATCCCCACGTTACCCCA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCATTTGGTCGAAGAAGGAGTGGGC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCATTTGGTCGAAGAAGGAGTGGGC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-PNCgl2766-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl2766-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_5",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_5",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=metH",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "metH",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_5",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_5",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCCTAAGAGGTGGCTTTTACGTCGTCAAG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCCTAAGAGGTGGCTTTTACGTCGTCAAG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCAGGTGCCCATGGCGCCGTC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCAGGTGCCCATGGCGCCGTC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-PmetH-venus",
"@type": [
"Sample"
],
"name": "pJC1-PmetH-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_6",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_6",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=NCgl1451",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "NCgl1451",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_6",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_6",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCCTACATCAGGGCGGGTGTCGTTG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCCTACATCAGGGCGGGTGTCGTTG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCATGTTGAGCCTTGCGGAGTGG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCATGTTGAGCCTTGCGGAGTGG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-PNCgl1451-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl1451-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_7",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_7",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=NCgl0176",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "NCgl0176",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_7",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_7",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCCTATCCAAACTCATCCGCTGCAGTTT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCCTATCCAAACTCATCCGCTGCAGTTT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCAACGGGCGGCGTCAGTTAAC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCAACGGGCGGCGTCAGTTAAC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-PNCgl0176-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl0176-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_8",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_8",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=NCgl0177",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "NCgl0177",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_8",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_8",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCCTAGGCGAGTACTTTGTCACGGG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCCTAGGCGAGTACTTTGTCACGGG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCAATCGTGGAGTCTTCTAAATTTTCGAGT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCAATCGTGGAGTCTTCTAAATTTTCGAGT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-PNCgl0177-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl0177-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_9",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_9",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=gyrB",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "gyrB",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_9",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_9",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCCTATGGGCCACCAATCTGCGCAT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCCTATGGGCCACCAATCTGCGCAT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCAAGCGTCATAATTGTGTTCAGTGTTTG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCAAGCGTCATAATTGTGTTCAGTGTTTG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-PgyrB-venus",
"@type": [
"Sample"
],
"name": "pJC1-PgyrB-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterPlasmidCloning_10",
"@type": [
"Process"
],
"name": "ReporterPlasmidCloning_10",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Gene of interest=wzy",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Gene of interest",
"categoryCode": ":",
"value": "wzy",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Plasmid backbone=pJC1-venus-term-BS",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Plasmid backbone",
"categoryCode": ":",
"value": "pJC1-venus-term-BS",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Backbone restriction=BamHI/BcuI",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Backbone restriction",
"categoryCode": ":",
"value": "BamHI/BcuI",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Insert Amplification=PCR",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Insert Amplification",
"categoryCode": ":",
"value": "PCR",
"valueCode": "http://purl.obolibrary.org/obo/C_34079",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strategy=Gibson Assembly",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strategy",
"categoryCode": ":",
"value": "Gibson Assembly",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Amplification of plasmid=Transformation of E. coli DH5a via heat shock",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Amplification of plasmid",
"categoryCode": ":",
"value": "Transformation of E. coli DH5a via heat shock",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Source_ReporterPlasmidCloning_Input_10",
"@type": [
"Source"
],
"name": "ReporterPlasmidCloning_Input_10",
"characteristics": [
{
"@id": "#MaterialAttributeValue/primer forward=AGCGACGCCGCAGGGGGATCCTAAAGCGGTCACCAGTTCGAC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward",
"categoryCode": ":",
"value": "AGCGACGCCGCAGGGGGATCCTAAAGCGGTCACCAGTTCGAC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse=ATGATATCTCCTTCTTAAAGTTCAACTTCCGACAGCTAAAGGCTCAC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse",
"categoryCode": ":",
"value": "ATGATATCTCCTTCTTAAAGTTCAACTTCCGACAGCTAAAGGCTCAC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer forward_venus insert=TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer forward_venus insert",
"categoryCode": ":",
"value": "TGAACTTTAAGAAGGAGATATCATATGGTGAGCAAGGGCGAGGA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/primer reverse_venus insert=AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "primer reverse_venus insert",
"categoryCode": ":",
"value": "AAAACGACGGCCAGTACTAGTTTACTTGTACAGCTCGTCCATGCC",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Template=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Template",
"categoryCode": "NCIT:C45677",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_pJC1-Pwzy-venus",
"@type": [
"Sample"
],
"name": "pJC1-Pwzy-venus",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_ReporterStrainTransformation",
"@type": [
"Process"
],
"name": "ReporterStrainTransformation",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Transformation method=Electroporation",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Transformation method",
"categoryCode": ":",
"value": "Electroporation",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_pJC1-PNCgl1781-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl1781-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PNCgl1781-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl1781-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PxerC-venus",
"@type": [
"Sample"
],
"name": "pJC1-PxerC-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PxerC-venus",
"@type": [
"Sample"
],
"name": "pJC1-PxerC-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-Pqor3-venus",
"@type": [
"Sample"
],
"name": "pJC1-Pqor3-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-Pqor3-venus",
"@type": [
"Sample"
],
"name": "pJC1-Pqor3-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PcepA-venus",
"@type": [
"Sample"
],
"name": "pJC1-PcepA-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PcepA-venus",
"@type": [
"Sample"
],
"name": "pJC1-PcepA-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PNCgl2766-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl2766-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PNCgl2766-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl2766-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PmetH-venus",
"@type": [
"Sample"
],
"name": "pJC1-PmetH-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PmetH-venus",
"@type": [
"Sample"
],
"name": "pJC1-PmetH-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PNCgl1451-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl1451-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PNCgl1451-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl1451-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PNCgl0176-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl0176-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PNCgl0176-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl0176-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PNCgl0177-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl0177-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PNCgl0177-venus",
"@type": [
"Sample"
],
"name": "pJC1-PNCgl0177-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PgyrB-venus",
"@type": [
"Sample"
],
"name": "pJC1-PgyrB-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-PgyrB-venus",
"@type": [
"Sample"
],
"name": "pJC1-PgyrB-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-Pwzy-venus",
"@type": [
"Sample"
],
"name": "pJC1-Pwzy-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=WT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "WT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_pJC1-Pwzy-venus",
"@type": [
"Sample"
],
"name": "pJC1-Pwzy-venus",
"additionalProperties": [
{
"@id": "#MaterialAttributeValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Parental strain=DdtxR",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Parental strain",
"categoryCode": ":",
"value": "DdtxR",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_WT-NCgl1781",
"@type": [
"Sample"
],
"name": "WT-NCgl1781",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-NCgl1781",
"@type": [
"Sample"
],
"name": "DdtxR-NCgl1781",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-xerC",
"@type": [
"Sample"
],
"name": "WT-xerC",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-xerC",
"@type": [
"Sample"
],
"name": "DdtxR-xerC",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-qor3",
"@type": [
"Sample"
],
"name": "WT-qor3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-qor3",
"@type": [
"Sample"
],
"name": "DdtxR-qor3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-cepA",
"@type": [
"Sample"
],
"name": "WT-cepA",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-cepA",
"@type": [
"Sample"
],
"name": "DdtxR-cepA",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-NCgl2766",
"@type": [
"Sample"
],
"name": "WT-NCgl2766",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-NCgl2766",
"@type": [
"Sample"
],
"name": "DdtxR-NCgl2766",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-metH",
"@type": [
"Sample"
],
"name": "WT-metH",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-metH",
"@type": [
"Sample"
],
"name": "DdtxR-metH",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-NCgl1451",
"@type": [
"Sample"
],
"name": "WT-NCgl1451",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-NCgl1451",
"@type": [
"Sample"
],
"name": "DdtxR-NCgl1451",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-NCgl0176",
"@type": [
"Sample"
],
"name": "WT-NCgl0176",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-NCgl0176",
"@type": [
"Sample"
],
"name": "DdtxR-NCgl0176",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-NCgl0177",
"@type": [
"Sample"
],
"name": "WT-NCgl0177",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-NCgl0177",
"@type": [
"Sample"
],
"name": "DdtxR-NCgl0177",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-gyrB",
"@type": [
"Sample"
],
"name": "WT-gyrB",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-gyrB",
"@type": [
"Sample"
],
"name": "DdtxR-gyrB",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_WT-wzy",
"@type": [
"Sample"
],
"name": "WT-wzy",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_DdtxR-wzy",
"@type": [
"Sample"
],
"name": "DdtxR-wzy",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Study": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"assays": "sdo:hasPart",
"filename": "sdo:alternateName",
"comments": "sdo:comment",
"processSequence": "sdo:about",
"studyDesignDescriptors": "arc:ARC#ARC_00000037"
}
},
{
"@id": "#study/2_ChAP-Seq",
"@type": [
"Study"
],
"additionalType": "Study",
"identifier": "2_ChAP-Seq",
"filename": "studies/2_ChAP-Seq/isa.study.xlsx",
"title": "Genome-wideBindingProfiling",
"description": "In order to evaluate on the genome-wide binding of DtxR and HrrA, chromatin-affinity purification coupled to sequencing (ChAP-seq) was performed.",
"people": [
{
"@id": "ai.krueger@fz-juelich.de",
"@type": "Person",
"orcid": "0000-0002-4172-2398",
"firstName": "Aileen",
"lastName": "Kr\u00fcger",
"email": "ai.krueger@fz-juelich.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_IBG-1:_Institute_of_Bio-_and_Geosciences,_Forschungszentrum_J\u00fclich_GmbH",
"name": "IBG-1: Institute of Bio- and Geosciences, Forschungszentrum J\u00fclich GmbH",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "ul.weber@fz-juelich.de",
"@type": "Person",
"firstName": "Ulrike",
"lastName": "Weber",
"email": "ul.weber@fz-juelich.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_IBG-1:_Institute_of_Bio-_and_Geosciences,_Forschungszentrum_J\u00fclich_GmbH",
"name": "IBG-1: Institute of Bio- and Geosciences, Forschungszentrum J\u00fclich GmbH",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
},
{
"@id": "j.frunzke@fz-juelich.de",
"@type": "Person",
"orcid": "0000-0001-6209-7950",
"firstName": "Julia",
"lastName": "Frunzke",
"email": "j.frunzke@fz-juelich.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_IBG-1:_Institute_of_Bio-_and_Geosciences,_Forschungszentrum_J\u00fclich_GmbH",
"name": "IBG-1: Institute of Bio- and Geosciences, Forschungszentrum J\u00fclich GmbH",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"processSequence": [
{
"@id": "#Process_Sample_preparation_0",
"@type": [
"Process"
],
"name": "Sample preparation_0",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::dtxR-C-linker-His",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::dtxR-C-linker-His",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=DtxR, C-terminal His Tag, including linker (2x GGGS)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "DtxR, C-terminal His Tag, including linker (2x GGGS)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=7.24",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "7.24",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_DtxR_iron1",
"@type": [
"Sample"
],
"name": "DtxR_iron1",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_DtxR_iron1_culture",
"@type": [
"Sample"
],
"name": "DtxR_iron1_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_1",
"@type": [
"Process"
],
"name": "Sample preparation_1",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::dtxR-C-linker-His",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::dtxR-C-linker-His",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=DtxR, C-terminal His Tag, including linker (2x GGGS)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "DtxR, C-terminal His Tag, including linker (2x GGGS)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=7.42",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "7.42",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_DtxR_iron2",
"@type": [
"Sample"
],
"name": "DtxR_iron2",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_DtxR_iron2_culture",
"@type": [
"Sample"
],
"name": "DtxR_iron2_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_2",
"@type": [
"Process"
],
"name": "Sample preparation_2",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::dtxR-C-linker-His",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::dtxR-C-linker-His",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=DtxR, C-terminal His Tag, including linker (2x GGGS)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "DtxR, C-terminal His Tag, including linker (2x GGGS)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=5.98",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "5.98",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_DtxR_iron3",
"@type": [
"Sample"
],
"name": "DtxR_iron3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_DtxR_iron3_culture",
"@type": [
"Sample"
],
"name": "DtxR_iron3_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_3",
"@type": [
"Process"
],
"name": "Sample preparation_3",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::hrrA-C-twinstrep",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::hrrA-C-twinstrep",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=HrrA, C-terminal Twinstrep Tag",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "HrrA, C-terminal Twinstrep Tag",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=7.68",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "7.68",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_HrrA_iron1",
"@type": [
"Sample"
],
"name": "HrrA_iron1",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_HrrA_iron1_culture",
"@type": [
"Sample"
],
"name": "HrrA_iron1_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_4",
"@type": [
"Process"
],
"name": "Sample preparation_4",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::hrrA-C-twinstrep",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::hrrA-C-twinstrep",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=HrrA, C-terminal Twinstrep Tag",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "HrrA, C-terminal Twinstrep Tag",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=6.72",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "6.72",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_HrrA_iron2",
"@type": [
"Sample"
],
"name": "HrrA_iron2",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_HrrA_iron2_culture",
"@type": [
"Sample"
],
"name": "HrrA_iron2_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_5",
"@type": [
"Process"
],
"name": "Sample preparation_5",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::hrrA-C-twinstrep",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::hrrA-C-twinstrep",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=HrrA, C-terminal Twinstrep Tag",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "HrrA, C-terminal Twinstrep Tag",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=6.12",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "6.12",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_HrrA_iron3",
"@type": [
"Sample"
],
"name": "HrrA_iron3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_HrrA_iron3_culture",
"@type": [
"Sample"
],
"name": "HrrA_iron3_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_6",
"@type": [
"Process"
],
"name": "Sample preparation_6",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::dtxR-C-linker-His",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::dtxR-C-linker-His",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=DtxR, C-terminal His Tag, including linker (2x GGGS)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "DtxR, C-terminal His Tag, including linker (2x GGGS)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=5.36",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "5.36",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_DtxR_heme1",
"@type": [
"Sample"
],
"name": "DtxR_heme1",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_DtxR_heme1_culture",
"@type": [
"Sample"
],
"name": "DtxR_heme1_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_7",
"@type": [
"Process"
],
"name": "Sample preparation_7",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::dtxR-C-linker-His",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::dtxR-C-linker-His",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=DtxR, C-terminal His Tag, including linker (2x GGGS)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "DtxR, C-terminal His Tag, including linker (2x GGGS)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=5.62",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "5.62",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_DtxR_heme2",
"@type": [
"Sample"
],
"name": "DtxR_heme2",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_DtxR_heme2_culture",
"@type": [
"Sample"
],
"name": "DtxR_heme2_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_8",
"@type": [
"Process"
],
"name": "Sample preparation_8",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::dtxR-C-linker-His",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::dtxR-C-linker-His",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=DtxR, C-terminal His Tag, including linker (2x GGGS)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "DtxR, C-terminal His Tag, including linker (2x GGGS)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=6.08",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "6.08",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_DtxR_heme3",
"@type": [
"Sample"
],
"name": "DtxR_heme3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_DtxR_heme3_culture",
"@type": [
"Sample"
],
"name": "DtxR_heme3_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_9",
"@type": [
"Process"
],
"name": "Sample preparation_9",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::hrrA-C-twinstrep",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::hrrA-C-twinstrep",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=HrrA, C-terminal Twinstrep Tag",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "HrrA, C-terminal Twinstrep Tag",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=5.92",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "5.92",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_HrrA_heme1",
"@type": [
"Sample"
],
"name": "HrrA_heme1",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_HrrA_heme1_culture",
"@type": [
"Sample"
],
"name": "HrrA_heme1_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_10",
"@type": [
"Process"
],
"name": "Sample preparation_10",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::hrrA-C-twinstrep",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::hrrA-C-twinstrep",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=HrrA, C-terminal Twinstrep Tag",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "HrrA, C-terminal Twinstrep Tag",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=6.06",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "6.06",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_HrrA_heme2",
"@type": [
"Sample"
],
"name": "HrrA_heme2",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_HrrA_heme2_culture",
"@type": [
"Sample"
],
"name": "HrrA_heme2_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_11",
"@type": [
"Process"
],
"name": "Sample preparation_11",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::hrrA-C-twinstrep",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::hrrA-C-twinstrep",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=HrrA, C-terminal Twinstrep Tag",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "HrrA, C-terminal Twinstrep Tag",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4 + 4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=4 \u00b5M hemin",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "4 \u00b5M hemin",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=6.38",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "6.38",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_HrrA_heme3",
"@type": [
"Sample"
],
"name": "HrrA_heme3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_HrrA_heme3_culture",
"@type": [
"Sample"
],
"name": "HrrA_heme3_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_12",
"@type": [
"Process"
],
"name": "Sample preparation_12",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::dtxR-C-linker-His",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::dtxR-C-linker-His",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=DtxR, C-terminal His Tag, including linker (2x GGGS)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "DtxR, C-terminal His Tag, including linker (2x GGGS)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=0 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "0 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=4.9",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "4.9",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_DtxR_starvation1",
"@type": [
"Sample"
],
"name": "DtxR_starvation1",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_DtxR_starvation1_culture",
"@type": [
"Sample"
],
"name": "DtxR_starvation1_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_13",
"@type": [
"Process"
],
"name": "Sample preparation_13",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::dtxR-C-linker-His",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::dtxR-C-linker-His",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=DtxR, C-terminal His Tag, including linker (2x GGGS)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "DtxR, C-terminal His Tag, including linker (2x GGGS)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=0 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "0 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=5.06",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "5.06",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_DtxR_starvation2",
"@type": [
"Sample"
],
"name": "DtxR_starvation2",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_DtxR_starvation2_culture",
"@type": [
"Sample"
],
"name": "DtxR_starvation2_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_14",
"@type": [
"Process"
],
"name": "Sample preparation_14",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::dtxR-C-linker-His",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::dtxR-C-linker-His",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=DtxR, C-terminal His Tag, including linker (2x GGGS)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "DtxR, C-terminal His Tag, including linker (2x GGGS)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=0 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "0 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=4.36",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "4.36",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_DtxR_starvation3",
"@type": [
"Sample"
],
"name": "DtxR_starvation3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_DtxR_starvation3_culture",
"@type": [
"Sample"
],
"name": "DtxR_starvation3_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_15",
"@type": [
"Process"
],
"name": "Sample preparation_15",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::hrrA-C-twinstrep",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::hrrA-C-twinstrep",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=HrrA, C-terminal Twinstrep Tag",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "HrrA, C-terminal Twinstrep Tag",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=0 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "0 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=4.62",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "4.62",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_HrrA_starvation1",
"@type": [
"Sample"
],
"name": "HrrA_starvation1",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_HrrA_starvation1_culture",
"@type": [
"Sample"
],
"name": "HrrA_starvation1_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_16",
"@type": [
"Process"
],
"name": "Sample preparation_16",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::hrrA-C-twinstrep",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::hrrA-C-twinstrep",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=HrrA, C-terminal Twinstrep Tag",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "HrrA, C-terminal Twinstrep Tag",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=0 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "0 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=4.61",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "4.61",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_HrrA_starvation2",
"@type": [
"Sample"
],
"name": "HrrA_starvation2",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_HrrA_starvation2_culture",
"@type": [
"Sample"
],
"name": "HrrA_starvation2_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_17",
"@type": [
"Process"
],
"name": "Sample preparation_17",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032::hrrA-C-twinstrep",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032::hrrA-C-twinstrep",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=HrrA, C-terminal Twinstrep Tag",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "HrrA, C-terminal Twinstrep Tag",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements without FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=0 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "0 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=4.18",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "4.18",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_HrrA_starvation3",
"@type": [
"Sample"
],
"name": "HrrA_starvation3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_HrrA_starvation3_culture",
"@type": [
"Sample"
],
"name": "HrrA_starvation3_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_18",
"@type": [
"Process"
],
"name": "Sample preparation_18",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032 (WT)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032 (WT)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=None",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "None",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=6.37",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "6.37",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_His_background1",
"@type": [
"Sample"
],
"name": "His_background1",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_His_background1_culture",
"@type": [
"Sample"
],
"name": "His_background1_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_19",
"@type": [
"Process"
],
"name": "Sample preparation_19",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032 (WT)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032 (WT)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=None",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "None",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=6.54",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "6.54",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_His_background2",
"@type": [
"Sample"
],
"name": "His_background2",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_His_background2_culture",
"@type": [
"Sample"
],
"name": "His_background2_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_20",
"@type": [
"Process"
],
"name": "Sample preparation_20",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032 (WT)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032 (WT)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=None",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "None",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=6.6",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "6.6",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_His_background3",
"@type": [
"Sample"
],
"name": "His_background3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_His_background3_culture",
"@type": [
"Sample"
],
"name": "His_background3_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_21",
"@type": [
"Process"
],
"name": "Sample preparation_21",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032 (WT)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032 (WT)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=None",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "None",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=6.52",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "6.52",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_Strep_background1",
"@type": [
"Sample"
],
"name": "Strep_background1",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_Strep_background1_culture",
"@type": [
"Sample"
],
"name": "Strep_background1_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_22",
"@type": [
"Process"
],
"name": "Sample preparation_22",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032 (WT)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032 (WT)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=None",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "None",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=5.59",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "5.59",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_Strep_background2",
"@type": [
"Sample"
],
"name": "Strep_background2",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_Strep_background2_culture",
"@type": [
"Sample"
],
"name": "Strep_background2_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#Process_Sample_preparation_23",
"@type": [
"Process"
],
"name": "Sample preparation_23",
"parameterValues": [
{
"@id": "#ProcessParameterValue/Organism=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Organism",
"categoryCode": "NCIT:C14250",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITaxon_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Strain=C. glutamicum ATCC13032 (WT)",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Strain",
"categoryCode": "NCIT:C95501",
"value": "C. glutamicum ATCC13032 (WT)",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/tag=None",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "tag",
"categoryCode": "MI:0507",
"value": "None",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Main cultivation condition=CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Main cultivation condition",
"categoryCode": ":",
"value": "CGXII + 2%(w/v) glucose + MgSO4 + CaCl2 + Biotin + PCA + trace elements with 100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/iron source=100 \u00b5M FeSO4",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "iron source",
"categoryCode": ":",
"value": "100 \u00b5M FeSO4",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Cultivation volume=5liter",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Cultivation volume",
"categoryCode": ":",
"value": 5.0,
"unit": "liter",
"unitCode": "https://bioregistry.io/UO:0000099",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/start OD600=3",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "start OD600",
"categoryCode": "DPBO:0000101",
"value": "3",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/end OD600=5.24",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "end OD600",
"categoryCode": ":",
"value": "5.24",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#ProcessParameterValue/Timepoint Harvesting=2hours",
"@type": "PropertyValue",
"additionalType": "ProcessParameterValue",
"category": "Timepoint Harvesting",
"categoryCode": ":",
"value": 2,
"unit": "hours",
"unitCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"inputs": [
{
"@id": "#Sample_Strep_background3",
"@type": [
"Sample"
],
"name": "Strep_background3",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_Strep_background3_culture",
"@type": [
"Sample"
],
"name": "Strep_background3_culture",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Study": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"assays": "sdo:hasPart",
"filename": "sdo:alternateName",
"comments": "sdo:comment",
"processSequence": "sdo:about",
"studyDesignDescriptors": "arc:ARC#ARC_00000037"
}
},
{
"@id": "#study/1_TagIntegration",
"@type": [
"Study"
],
"additionalType": "Study",
"identifier": "1_TagIntegration",
"filename": "studies/1_TagIntegration/isa.study.xlsx",
"title": "Tagging DtxR",
"description": "In order to perform ChAP-Seq, it was necessary to gain a tagged variant of DtxR. Previous studies within our lab recognized that a normal tagging both C- or N-terminal disturb DtxR DNA binding ability. Therefore a linker sequence should be added between DtxR and a respective His-Tag (Chen et al., 2013). This should be tested both C- and N-terminal and the variant that does not disturb growth phenotype as well as expression should be used for the big ChAP-Seq approach.",
"people": [
{
"@id": "ai.krueger@fz-juelich.de",
"@type": "Person",
"orcid": "0000-0001-7060-4879",
"firstName": "Kr\u00fcger",
"lastName": "Aileen",
"email": "ai.krueger@fz-juelich.de",
"affiliation": {
"@type": "Organization",
"@id": "#Organization_Forschungszentrum_J\u00fclich_GmbH",
"name": "Forschungszentrum J\u00fclich GmbH",
"@context": {
"sdo": "http://schema.org/",
"Organization": "sdo:Organization",
"name": "sdo:name"
}
},
"@context": {
"sdo": "http://schema.org/",
"Person": "sdo:Person",
"orcid": "sdo:identifier",
"firstName": "sdo:givenName",
"lastName": "sdo:familyName",
"midInitials": "sdo:additionalName",
"email": "sdo:email",
"address": "sdo:address",
"phone": "sdo:telephone",
"fax": "sdo:faxNumber",
"comments": "sdo:disambiguatingDescription",
"roles": "sdo:jobTitle",
"affiliation": "sdo:affiliation"
}
}
],
"processSequence": [
{
"@id": "#Process_StrainConstruction",
"@type": [
"Process"
],
"name": "StrainConstruction",
"inputs": [
{
"@id": "#Source_StrainConstruction_Input_0",
"@type": [
"Source"
],
"name": "StrainConstruction_Input_0",
"characteristics": [
{
"@id": "#MaterialAttributeValue/parental strain=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "parental strain",
"categoryCode": ":",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/integration plasmid=pK19-mobsacB-DtxR-His-C-2link",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "integration plasmid",
"categoryCode": ":",
"value": "pK19-mobsacB-DtxR-His-C-2link",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Tag=CATCATCATCATCATCATCATCATCATCAT",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Tag",
"categoryCode": ":",
"value": "CATCATCATCATCATCATCATCATCATCAT",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/linker=GGCGGCGGCGGCTCAGGCGGCGGCGGCTCA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "linker",
"categoryCode": ":",
"value": "GGCGGCGGCGGCTCAGGCGGCGGCGGCTCA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/terminus of tag=C",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "terminus of tag",
"categoryCode": ":",
"value": "C",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
},
{
"@id": "#Source_StrainConstruction_Input_1",
"@type": [
"Source"
],
"name": "StrainConstruction_Input_1",
"characteristics": [
{
"@id": "#MaterialAttributeValue/parental strain=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "parental strain",
"categoryCode": ":",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/integration plasmid=pK19-mobsacB-DtxR-Strep-N-4link",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "integration plasmid",
"categoryCode": ":",
"value": "pK19-mobsacB-DtxR-Strep-N-4link",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Tag=TGGTCCCACCCACAGTTCGAGAAG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Tag",
"categoryCode": ":",
"value": "TGGTCCCACCCACAGTTCGAGAAG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/linker=GGCGGCGGCGGCTCAGGCGGCGGCGGCTCAGGCGGCGGCGGCTCAGGCGGCGGCGGCTCA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "linker",
"categoryCode": ":",
"value": "GGCGGCGGCGGCTCAGGCGGCGGCGGCTCAGGCGGCGGCGGCTCAGGCGGCGGCGGCTCA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/terminus of tag=N",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "terminus of tag",
"categoryCode": ":",
"value": "N",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
},
{
"@id": "#Source_StrainConstruction_Input_2",
"@type": [
"Source"
],
"name": "StrainConstruction_Input_2",
"characteristics": [
{
"@id": "#MaterialAttributeValue/parental strain=Corynebacterium glutamicum ATCC 13032",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "parental strain",
"categoryCode": ":",
"value": "Corynebacterium glutamicum ATCC 13032",
"valueCode": "http://purl.obolibrary.org/obo/NCBITAXON_196627",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/integration plasmid=pK19-mobsacB-DtxR-Strep-N-2link",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "integration plasmid",
"categoryCode": ":",
"value": "pK19-mobsacB-DtxR-Strep-N-2link",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/Tag=TGGTCCCACCCACAGTTCGAGAAG",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "Tag",
"categoryCode": ":",
"value": "TGGTCCCACCCACAGTTCGAGAAG",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/linker=GGCGGCGGCGGCTCAGGCGGCGGCGGCTCA",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "linker",
"categoryCode": ":",
"value": "GGCGGCGGCGGCTCAGGCGGCGGCGGCTCA",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
},
{
"@id": "#MaterialAttributeValue/terminus of tag=N",
"@type": "PropertyValue",
"additionalType": "MaterialAttributeValue",
"category": "terminus of tag",
"categoryCode": ":",
"value": "N",
"valueCode": "",
"@context": {
"sdo": "http://schema.org/",
"additionalType": "sdo:additionalType",
"alternateName": "sdo:alternateName",
"measurementMethod": "sdo:measurementMethod",
"description": "sdo:description",
"category": "sdo:name",
"categoryCode": "sdo:propertyID",
"value": "sdo:value",
"valueCode": "sdo:valueReference",
"unit": "sdo:unitText",
"unitCode": "sdo:unitCode",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Source": "bio:Sample",
"name": "sdo:name",
"characteristics": "bio:additionalProperty"
}
}
],
"outputs": [
{
"@id": "#Sample_C._glutamicum::dtxR-His-Cterm-2link",
"@type": [
"Sample"
],
"name": "C. glutamicum::dtxR-His-Cterm-2link",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_C._glutamicum::dtxR-Strep-Nterm-4link",
"@type": [
"Sample"
],
"name": "C. glutamicum::dtxR-Strep-Nterm-4link",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
},
{
"@id": "#Sample_C._glutamicum::dtxR-Strep-Nterm-2link",
"@type": [
"Sample"
],
"name": "C. glutamicum::dtxR-Strep-Nterm-2link",
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Sample": "bio:Sample",
"name": "sdo:name",
"additionalProperties": "bio:additionalProperty"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"bio": "https://bioschemas.org/",
"Process": "bio:LabProcess",
"name": "sdo:name",
"executesProtocol": "bio:executesLabProtocol",
"parameterValues": "bio:parameterValue",
"performer": "sdo:agent",
"date": "sdo:endTime",
"inputs": "sdo:object",
"outputs": "sdo:result",
"comments": "sdo:disambiguatingDescription"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Study": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"assays": "sdo:hasPart",
"filename": "sdo:alternateName",
"comments": "sdo:comment",
"processSequence": "sdo:about",
"studyDesignDescriptors": "arc:ARC#ARC_00000037"
}
}
],
"@context": {
"sdo": "http://schema.org/",
"Investigation": "sdo:Dataset",
"identifier": "sdo:identifier",
"title": "sdo:headline",
"additionalType": "sdo:additionalType",
"description": "sdo:description",
"submissionDate": "sdo:dateCreated",
"publicReleaseDate": "sdo:datePublished",
"publications": "sdo:citation",
"people": "sdo:creator",
"studies": "sdo:hasPart",
"ontologySourceReferences": "sdo:mentions",
"comments": "sdo:comment",
"filename": "sdo:alternateName"
}
},
"conformsTo": {
"@id": "https://w3id.org/ro/crate/1.1"
},
"@context": {
"sdo": "http://schema.org/",
"arc": "http://purl.org/nfdi4plants/ontology/",
"CreativeWork": "sdo:CreativeWork",
"about": "sdo:about",
"conformsTo": "sdo:conformsTo"
}
}